Heterologous Prime-Boost Vaccination with Inactivated Influenza Viruses Induces More Effective Cross-Protection than Homologous Repeat Vaccination

With concerns about the efficacy of repeat annual influenza vaccination, it is important to better understand impact priming vaccine immunity and develop an effective vaccination strategy. Here, we determined heterologous prime-boost on inducing broader protective compared with same antigen. The primed mice that were intramuscularly boosted a inactivated A virus (H1N1, H3N2, H5N1, H7N9, H9N2) showed increased strain-specific hemagglutination inhibition titers against prime boost strains. Heterologous viruses was more in high levels IgG antibodies specific for groups 1 2 hemagglutinin stalk domains, as well cross-protection, homologous vaccination. Both humoral T cell found play critical role conferring cross-protection by These results support strategy enhance cross-protective